Gina jiki denaturation

Conformation (tsarin) na peptide sarkar da aka yi umurni da shi ne musamman domin kowane sunadari. A yanayi na musamman akwai rata manyan yawan shaidu wanda dattako da na sarari tsarin da fili kwayoyin. A sakamakon haka, da rata gaba daya da dukkan kwayoyin (ko da wani gwaji rabo daga gare shi) daukan nau'i na bazuwar nada. Wannan tsari shine ake kira "denaturation". Wannan canji iya fararwa zafi daga sittin zuwa tamanin digiri. Saboda haka, duk kwayoyin, sakamakon gibin na iya bambanta a conformation daga wasu.

Denaturation sunadaran faruwa a ƙarƙashin rinjayar da wani jamiái iya lalata ba covalent shaidu. Wannan tsari na iya faruwa a karkashin alkaline ko acidic yanayi, a kan surface na zamani sashe, a karkashin mataki na wasu kwayoyin mahadi (phenols, alcohols da sauransu). gina jiki denaturation iya faruwa a ƙarƙashin rinjayar guanidinium chloride ko urea. Ya ce jamiái samar da rauni shaidu (hydrophobic, ionic, hydrogen) tare da carbonyl ko amino kungiyoyin na peptide kashin da yawan kungiyoyin na amino acid sharan, musanya nasu samuwa gina jiki hydrogen shaidu a cikin kwayoyin. A sakamakon haka, a canji yakan faru a cikin sakandare da kuma manyan tsarin.

Resistance zuwa denaturing jamiái dogara, yafi a kan gaban a kwayoyin na gina jiki mahadi disulfide shaidu. Trypsin hanawa yana da uku shaidu S - S. karkashin yanayin dawo da furotin denaturation auku ba tare da sauran tsoma. Idan baya sa da connection a cikin yanayi inda da hadawan abu da iskar shaka ne da za'ayi SH-kungiyoyin na cysteine da kuma samuwar disulfide shaidu, da asali conformation aka mayar. A gaban ko da guda disulfide bond muhimmanci ƙara da zaman lafiyar na sarari tsarin.

gina jiki denaturation, yawanci tare da wani karu a solubility. Tare da wannan precipitate sau da yawa siffofin. Yana auku a cikin nau'i na "curdled gina jiki." A high yawa daga cikin mahadi a cikin bayani, "coagulation" shigarsu dukan bayani, kamar yadda, misali, wannan ya faru a lokacin da ruwan qwai. Lokacin da denaturation na gina jiki hasarar da nazarin halittu aiki. Wannan manufa ne dangane da yin amfani da carbolic acid (phenol ruwa-ruwa bayani) a matsayin antiseptic abu.

A rashin zaman lafiya da na sarari tsarin, babban Yiwuwar gazawar ƙarƙashin rinjayar daban-daban jamiái ne muhimmanci wuya a ware da kuma gina jiki gudanar da bincike. Kuma Ya halitta wasu matsaloli a lokacin da amfani da mahadi a magani da kuma masana'antu.

Idan gina jiki denaturation da aka za'ayi da daukan hotuna zuwa high yanayin zafi, jinkirin sanyaya faruwa a wasu yanayi da tsari renativatsii - dawo da 'yan qasar (asali) conformation. Wannan hujja itace ta tabbatar da cewa kwanciya daga cikin peptide sarkar ne daidai da primary tsarin.

Da samuwar 'yan qasar conformation (source location) ne wani yunkri tsari. A wasu kalmomin, wannan tsari yayi dace da mafi ƙarancin adadin free makamashi kunshe ne a cikin wani kwayoyin. Daya iya a sakamakon da za a kammala da cewa da na sarari tsarin da fili da aka shigar wanda ke aiki a cikin jerin amino acid a cikin peptide sarƙoƙi. Wannan bi da bi yana nufin cewa duk polypeptides alaka da canzawar amino acid (misali, mioglobinovye peptide sarƙoƙi), zai dauki wani m conformation.

Sunadaran iya zama gagarumin bambance-bambance a cikin primary tsarin, ko da idan kadan ko cikakken m, a conformation.